MiR-21-5p but not miR-1-3p expression is modulated by preconditioning in a rat model of myocardial infarction.
Annika RaupachCarolin TorregrozaJulia NiesteggeKatharina FeigeSwantje Klemm-MeyerInge BauerTimo BrandenburgerHilbert GrievinkAndré HeinenRagnar HuhnPublished in: Molecular biology reports (2020)
Isoflurane (Iso) preconditioning (PC) is known to be cardioprotective against ischemia/reperfusion (I/R) injury. It was previously shown that microRNA-21-5p (miR-21-5p) is regulated by Iso-PC. It is unclear, if expression of cardiac enriched miR-1-3p is also affected by Iso-PC, and associated with activation of HIF1α (hypoxia-inducible factor 1-alpha). Male Wistar rats (n = 6-8) were randomly assigned to treatment with or without 1 MAC Iso for 30 min, followed by 25 min of regional myocardial ischemia, with 120 min reperfusion. At the end of reperfusion, myocardial expression of miR-1-3p, miR-21-5p and mRNAs of two HIF-1α-dependent genes, VEGF (vascular endothelial growth factor) and HO-1 (heme oxygenase-1), were determined by quantitative PCR. Protein expression of a miR-21 target gene, PDCD4 (programmed cell death protein 4), was assessed by western blot analysis. Infarct sizes were analyzed with triphenyltetrazoliumchloride staining. MiR-21-5p expression was increased by Iso, whereas expression of miR-1-3p was not altered. The expression of VEGF but not HO-1 was induced by Iso. Iso-PC reduced infarct sizes compared to untreated controls. No regulation of miRNA and mRNA expression was detected after I/R. PDCD4 protein expression was not affected after Iso exposure. Expression of miR-21-5p, in contrast to miR-1-3p, is altered during this early time point of Iso-PC. HIF1α signaling seems to be involved in miR-21-5p regulation.
Keyphrases
- poor prognosis
- vascular endothelial growth factor
- binding protein
- long non coding rna
- endothelial cells
- left ventricular
- acute myocardial infarction
- heart failure
- cell proliferation
- high resolution
- magnetic resonance
- computed tomography
- magnetic resonance imaging
- gene expression
- acute coronary syndrome
- genome wide
- mass spectrometry
- brain injury
- coronary artery disease
- signaling pathway
- contrast enhanced
- amino acid
- protein protein
- data analysis
- genome wide identification