Tissue-resident CXCR4 + macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression.
Zhenyu LiaoLongyun YeTianjiao LiXing JinXuan LinQinglin FeiHuiru ZhangSaimeng ShiXianjun YuKaizhou JinWei-Ding WuPublished in: International journal of cancer (2023)
Macrophage is an essential part of the tumor immune microenvironment of pancreatic ductal adenocarcinoma. In this study, we explored the CXCR4 + macrophages subset on its prognosis value, immune profile and distinct function in pancreatic cancer progression. Specimens from 102 postoperative pancreatic patients were analyzed by flow cytometry or immune-fluorescence, and the prognostic value of CXCR4 + macrophages infiltration was further determined by Cox regression. In silico analysis on TCGA, ICGC database and single-cell sequencing of pancreatic ductal adenocarcinoma further validated our findings. We found that high CXCR4 + macrophages infiltration was associated with poor overall survival (p < .01) and disease-free survival (p < .05) as an independent factor. CXCR4 + macrophages exhibited an M2 pro-tumor phenotype with high expression of CD206. The function of CXCR4 + macrophages was further analyzed in the murine orthotopic PDAC model with its tumor promotion effect and inhibition of CD8 + T cells. Mechanistic and RNA-seq analysis showed that CXCR4 + macrophages participated in extracellular matrix remodeling procedures and especially secreted SPARC through CXCR4/PI3K/Akt pathway promoting tumor proliferation and migration. Our study reveals that CXCR4 + macrophages infiltration is an indicator of poor prognosis of PDAC and targeting these cells was potentially crucial in immunotherapy of PDAC. This article is protected by copyright. All rights reserved.
Keyphrases
- poor prognosis
- single cell
- rna seq
- cell migration
- long non coding rna
- extracellular matrix
- free survival
- flow cytometry
- adipose tissue
- stem cells
- patients undergoing
- emergency department
- induced apoptosis
- patient safety
- cell death
- cancer therapy
- oxidative stress
- cell proliferation
- endoplasmic reticulum stress
- quality improvement
- molecular dynamics simulations
- electronic health record