Mass spectrometry-based metabolomics in microbiome investigations.
Anelize BauermeisterHelena M RussoLeticia Veras Costa-LotufoAlan K JarmuschPieter C DorresteinPublished in: Nature reviews. Microbiology (2021)
Microbiotas are a malleable part of ecosystems, including the human ecosystem. Microorganisms affect not only the chemistry of their specific niche, such as the human gut, but also the chemistry of distant environments, such as other parts of the body. Mass spectrometry-based metabolomics is one of the key technologies to detect and identify the small molecules produced by the human microbiota, and to understand the functional role of these microbial metabolites. This Review provides a foundational introduction to common forms of untargeted mass spectrometry and the types of data that can be obtained in the context of microbiome analysis. Data analysis remains an obstacle; therefore, the emphasis is placed on data analysis approaches and integrative analysis, including the integration of microbiome sequencing data.
Keyphrases
- mass spectrometry
- data analysis
- liquid chromatography
- endothelial cells
- induced pluripotent stem cells
- gas chromatography
- capillary electrophoresis
- high resolution
- high performance liquid chromatography
- pluripotent stem cells
- climate change
- lymph node
- big data
- microbial community
- high resolution mass spectrometry
- single cell
- ms ms