Sustainable production of single-cell oil and protein from wastepaper hydrolysate: identification and optimization of a Rhodotorula mucilaginosa strain as a promising yeast.
Amador Campos-ValdezManuel R KirchmayrIliana Barrera MartínezLeticia Casas GodoyPublished in: FEMS yeast research (2023)
This study investigated the potential of wastepaper hydrolysate as a sustainable and low-cost carbon source for single-cell oil and protein production, attending to the growing need for alternative feedstocks and waste management strategies. Wastepaper, characterized by its high carbohydrate content, was subjected to enzymatic and chemo-enzymatic treatments for carbohydrate release. The chemo-enzymatic treatment performed better, yielding 65.3 g L-1 of fermentable sugars. Sixty-two yeast strains were screened for single-cell oil accumulation, identifying Rhodotorula mucilaginosa M1K4 as the most advantageous oleaginous yeast. M1K4 lipid production was optimized in liquid culture, and its fatty acid profile was analyzed, showing a high content of industrially valuable fatty acids, particularly palmitic (28%) and oleic (51%). Batch-culture of M1K4 in a 3 L reactor demonstrated the strain's ability to utilize wastepaper hydrolysate as a carbon source, with dry cell weight, total lipid and protein production of 17.7 g L-1, 4.5 g L-1, and 2.1 g L-1, respectively. Wastepaper as a substrate provides a sustainable solution for waste management and bioproduction. This research highlights the potential of R. mucilaginosa for lipid and protein production from wastepaper hydrolysate.
Keyphrases
- fatty acid
- single cell
- rna seq
- protein protein
- amino acid
- low cost
- hydrogen peroxide
- high throughput
- escherichia coli
- photodynamic therapy
- physical activity
- binding protein
- saccharomyces cerevisiae
- radiation therapy
- combination therapy
- mesenchymal stem cells
- nitric oxide
- mass spectrometry
- cell wall
- cell therapy
- drug delivery
- small molecule
- locally advanced
- anaerobic digestion
- climate change
- weight gain
- atomic force microscopy
- replacement therapy