Satureja bachtiarica Induces Cancer Cell Death in Breast and Glioblastoma Cancer in 2D/3D Models and Suppresses Breast Cancer Stem Cells.
Vajihe Azimian ZavarehShima GharibiMahnaz HosseiniAbdolhossein NekookarHossein MirhendiMehdi RahimmalekAntoni SzumnyPublished in: Cells (2023)
Overcoming drug resistance and specifically targeting cancer stem cells (CSCs) are critical challenges in improving cancer therapy. Nowadays, the use of novel and native medicinal plants can provide new sources for further investigations for this purpose. The aim of this study was to assess the potential of S. bachtiarica , an endemic plant with diverse medicinal applications, in suppressing and targeting cancer and cancer stem cells in glioblastoma and breast cancer. The effect of S. bachtiarica on viability, migration, invasion, and clonogenic potential of MDAMB-231 and U87-MG cells was assessed in both two- and three-dimensional cell culture models. Additionally, we evaluated its effects on the self-renewal capacity of mammospheres. The experimental outcomes indicated that S. bachtiarica decreased the viability and growth rate of cells and spheroids by inducing apoptosis and inhibited colony formation, migration, and invasion of cells and spheroids. Additionally, colony and sphere-forming ability, as well as the expression of genes associated with EMT and stemness were reduced in mammospheres treated with S. bachtiarica . In conclusion, this study provided valuable insights into the anti-cancer effects of S. bachtiarica , particularly in relation to breast CSCs. Therefore, S. bachtiarica may be a potential adjuvant for the treatment of cancer.
Keyphrases
- cancer stem cells
- cell cycle arrest
- papillary thyroid
- cell death
- induced apoptosis
- cancer therapy
- squamous cell
- endoplasmic reticulum stress
- epithelial mesenchymal transition
- signaling pathway
- oxidative stress
- poor prognosis
- drug delivery
- pi k akt
- stem cells
- early stage
- insulin resistance
- squamous cell carcinoma
- childhood cancer
- long non coding rna
- adipose tissue
- type diabetes
- cell migration
- skeletal muscle
- binding protein
- smoking cessation