Lignan from Alnus japonica Inhibits Adipocyte Differentiation via Cell Cycle and FOXO1 Regulation.
Hyejin LeeJi Hye JeongJae-Ha RyuPublished in: Molecules (Basel, Switzerland) (2020)
In the present study, we isolated a lignan ((-)-(2R,3R)-1,4-O-diferuloylsecoisolariciresinol, DFS) from Alnus japonica and evaluated its antiobesity potential in vitro. We also determined its mechanism of action in a mouse pre-adipocyte 3T3-L1 cell line. DFS dose- and day-dependently inhibited adipogenesis by downregulation of adipogenic factors and lipid metabolism-regulating factors during adipocyte differentiation. In particular, DFS suppressed cell cycle-regulating factors and induced G0/G1 cell cycle arrest, implying that it had an inhibitory effect on mitotic clonal expansion which occurred at an early stage of adipogenesis. DFS also suppressed adipogenesis through decreasing Akt phosphorylation and increasing the level of Forkhead box protein-O1 (FOXO1). These results suggest that DFS may be a pharmacological candidate for the development of antiobesity, therapeutic, and nutraceutical products.
Keyphrases
- cell cycle
- cell proliferation
- pi k akt
- transcription factor
- cell cycle arrest
- signaling pathway
- adipose tissue
- early stage
- fatty acid
- insulin resistance
- cell death
- high fat diet induced
- type diabetes
- high glucose
- squamous cell carcinoma
- metabolic syndrome
- skeletal muscle
- radiation therapy
- rectal cancer
- diabetic rats
- sentinel lymph node
- protein kinase