Lipid-Lowering Effects of a Novel Polysaccharide Obtained from Fuzhuan Brick Tea In Vitro.
Wenjuan YangShirui ChengMeng LiuNan LiJing WangWen-Bo YaoFuxin ChenJian-Wu XiePin GongPublished in: Foods (Basel, Switzerland) (2023)
Lipid accumulation causes diseases such as obesity and abnormal lipid metabolism, thus impairing human health. Tea polysaccharide is one of the natural, active substances that can lower lipid levels. In this paper, an oleic-acid-induced HepG2 cell model was established. The lipid-lowering effects of a novel group of Fuzhuan brick tea polysaccharides (FTPs)-obtained from Fuzhuan brick tea-were examined in vitro. The monosaccharide composition of FTP3 was Glc, Gal, Ara, Man, Rha, GalAc, GlcAc, and Xyl with a molar ratio of 23.5:13.2:9.0:5.5:5.4:2.7:1.3:1.0, respectively. A molecular weight of 335.68 kDa was identified for FTP3. HepG2 cells treated with FTP3 achieved a prominent lipid-lowering effect compared with cells treated with oleic acid. Images of the Oil Red O staining treatment showed that FTP3-treated groups had significantly fewer red fat droplets. TC and TG levels were lower in FTP3-treated groups. FTP3 alleviated lipid accumulation in HepG2 cells, activated AMPK, and decreased the SREBP-1C and FAS protein expressions associated with fatty acid synthesis. FTP3 holds promising potential for its lipid-lowering effects.
Keyphrases
- fatty acid
- human health
- risk assessment
- metabolic syndrome
- induced apoptosis
- climate change
- adipose tissue
- insulin resistance
- deep learning
- weight loss
- single cell
- newly diagnosed
- cell therapy
- stem cells
- oxidative stress
- physical activity
- cell death
- cell cycle arrest
- mesenchymal stem cells
- heat shock protein
- combination therapy