SPARC enables genetic manipulation of precise proportions of cells.
Jesse Isaacman-BeckKristine C PaikCarl F R WieneckeHelen H YangYvette E FisherIrving E WangItzel G IshidaGaby MaimonRachel I WilsonThomas R ClandininPublished in: Nature neuroscience (2020)
Many experimental approaches rely on controlling gene expression in select subsets of cells within an individual animal. However, reproducibly targeting transgene expression to specific fractions of a genetically defined cell type is challenging. We developed Sparse Predictive Activity through Recombinase Competition (SPARC), a generalizable toolkit that can express any effector in precise proportions of post-mitotic cells in Drosophila. Using this approach, we demonstrate targeted expression of many effectors in several cell types and apply these tools to calcium imaging of individual neurons and optogenetic manipulation of sparse cell populations in vivo.
Keyphrases
- induced apoptosis
- gene expression
- cell cycle arrest
- poor prognosis
- single cell
- endoplasmic reticulum stress
- high resolution
- oxidative stress
- cell therapy
- immune response
- cell death
- drug delivery
- spinal cord
- bone marrow
- cell proliferation
- copy number
- spinal cord injury
- dendritic cells
- regulatory t cells
- mass spectrometry
- binding protein
- long non coding rna
- photodynamic therapy
- pi k akt