Click-Chemistry Cross-Linking of Hyaluronan Graft Copolymers.

An easy and viable crosslinking procedure by click-chemistry (click-crosslinking) of hyaluronic acid ( HA ) was developed. In particular, the clickable propargyl groups of hyaluronane-based HA - FA - Pg graft copolymers showing low and medium molecular weight values were exploited in crosslinking by click-chemistry by using a hexa(ethylene glycol) spacer. The resulting HA-FA-HEG-CL materials showed an apparent lack of in vitro cytotoxic effects, tuneable water affinity, and rheological properties according to the crosslinking degree that suggests their applicability in different biomedical fields.