FACS to Identify Immune Subsets in Mouse Brain and Spleen.
Mary K MaloneThomas A UjasKatherine M CotterDaimen R S BritschJenny LutshumbaJadwiga Turchan-CholewoAnn M StowePublished in: Methods in molecular biology (Clifton, N.J.) (2023)
Flow cytometry enables the multi-parametric quantification of cell types, especially in immunophenotyping of unique immune cell subsets that can either contribute to or ameliorate pathology. For tissues to be used in such analyses, single-cell suspensions must be created. Here we describe protocols for preparing single-cell suspensions of mouse spleen and brain tissue, as well as the steps for fluorescently activated cell staining/sorting (FACS). Specifically, this protocol enables the isolation of lymphocytes for the study of immune responses during various diseases, such as long-term neuroinflammation following ischemic stroke.
Keyphrases
- single cell
- flow cytometry
- rna seq
- immune response
- peripheral blood
- high throughput
- gene expression
- randomized controlled trial
- cell therapy
- traumatic brain injury
- atrial fibrillation
- multiple sclerosis
- cerebral ischemia
- bone marrow
- blood brain barrier
- lps induced
- inflammatory response
- mesenchymal stem cells
- brain injury
- subarachnoid hemorrhage