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Dual DNA/RNA-binding factor regulates dynamics of hnRNP splicing condensates.

Mukulika RayJulia ZaborowskyPranav MahableshwarkarSmriti VaidyanathanJasmine ShumRenjith ViswanathanAnnie HuangSzu-Huan WangVictoria JohnsonNoah WakeAshley M ConardAlexander E ConicellaRyan PuterbaughNicolas L FawziErica Larschan
Published in: bioRxiv : the preprint server for biology (2024)
Despite decades of research, mechanisms by which co-transcriptional alternative splicing events are targeted to the correct genomic locations to drive cell fate decisions remain unknown. By combining structural and molecular approaches, we define a new mechanism by which an essential transcription factor (TF) targets co-transcriptional splicing through physical and functional interaction with RNA and RNA binding proteins (RBPs). We show that an essential TF co-transcriptionally regulates sex-specific alternative splicing by directly interacting with a subset of target RNAs on chromatin and modulating the dynamics of hnRNPA2 homolog nuclear splicing condensates.
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