Cannabidiol as Self-Assembly Inducer for Anticancer Drug-Based Nanoparticles.
Eleonora ColomboDavide Andrea CoppiniLaura PolitoUmberto CirielloGiuseppe PaladinoMariafrancesca HyeraciMaria Luisa Di PaoloGiulia NordioLisa Dalla ViaDaniele PassarellaPublished in: Molecules (Basel, Switzerland) (2022)
Cannabidiol (CBD) is a biologically active compound present in the plants of the Cannabis family, used as anticonvulsant, anti-inflammatory, anti-anxiety, and more recently, anticancer drug. In this work, its use as a new self-assembly inducer in the formation of nanoparticles is validated. The target conjugates are characterized by the presence of different anticancer drugs (namely N -desacetyl thiocolchicine, podophyllotoxin, and paclitaxel) connected to CBD through a linker able to improve drug release. These nanoparticles are formed via solvent displacement method, resulting in monodisperse and stable structures having hydrodynamic diameters ranging from 160 to 400 nm. Their biological activity is evaluated on three human tumor cell lines (MSTO-211H, HT-29, and HepG2), obtaining GI 50 values in the low micromolar range. Further biological assays were carried out on MSTO-211H cells for the most effective NP 8B , confirming the involvement of paclitaxel in cytotoxicity and cell death mechanism.
Keyphrases
- drug release
- cell death
- cell cycle arrest
- anti inflammatory
- induced apoptosis
- endothelial cells
- photodynamic therapy
- walled carbon nanotubes
- high resolution
- drug induced
- ionic liquid
- oxidative stress
- adverse drug
- emergency department
- endoplasmic reticulum stress
- signaling pathway
- cancer therapy
- induced pluripotent stem cells
- sleep quality
- cell proliferation
- pi k akt
- pluripotent stem cells
- light emitting