miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma.
Xiaoxiao ZhangChengyong YangZhen MengHuanhuan ZhongXutian HouFenfen WangYiping LuJingjing GuoYan ZengPublished in: International journal of molecular sciences (2023)
Neuroblastoma (NB) is the most common extracranial solid tumor that affects developing nerve cells in the fetus, infants, and children. miR-124 is a microRNA (miRNA) enriched in neuronal tissues, and VAMP3 (vesicle-associated membrane protein 3) has been reported to be an miR-124 target, although the relationship between NB and miR-124 or VAMP3 is unknown. Our current work identified that miR-124 levels are high in NB cases and that elevated miR-124 correlates with worse NB outcomes. Conversely, depressed VAMP3 correlates with worse NB outcomes. To investigate the mechanisms by which miR-124 and VAMP3 regulate NB, we altered miR-124 or VAMP3 expression in human NB cells and observed that increased miR-124 and reduced VAMP3 stimulated cell proliferation and suppressed apoptosis, while increased VAMP3 had the opposite effects. Genome-wide mRNA expression analyses identified gene and pathway changes which might explain the NB cell phenotypes. Together, our studies suggest that miR-124 and VAMP3 could be potential new markers of NB and targets of NB treatments.
Keyphrases
- cell proliferation
- long non coding rna
- long noncoding rna
- poor prognosis
- genome wide
- endothelial cells
- cell cycle arrest
- induced apoptosis
- type diabetes
- gene expression
- stem cells
- endoplasmic reticulum stress
- risk assessment
- young adults
- oxidative stress
- transcription factor
- single cell
- copy number
- binding protein
- induced pluripotent stem cells