Effect of Esculetin on Tert -Butyl Hydroperoxide-Induced Oxidative Injury in Retinal Pigment Epithelial Cells In Vitro.
Woo Kwon JungSu-Bin ParkHwa Young YuYong Hwan KimJunghyun KimPublished in: Molecules (Basel, Switzerland) (2022)
Esculetin is a coumarin-derived compound with antioxidant and anti-inflammatory properties. The current study aims to evaluate the therapeutic implications of esculetin on retinal dysfunction and uncover the underlying mechanisms. Tert -butyl hydroperoxide ( t -BHP) at a concentration of 300 μM was used to induce oxidative stress in human retinal pigment epithelial cell line (ARPE-19) cells. Esculetin at concentrations below 250 μM did not cause cytotoxicity to ARPE-19 cells. Cell viability analysis confirmed that t -BHP induced oxidative injury of ARPE-19 cells. However, ARPE-19 cells were protected from t -BHP-induced oxidative injury by esculetin in a concentration-dependent manner. As a result of the TUNEL assay to confirm apoptosis, esculetin treatment reduced the number of TUNEL-positive cells. Esculetin down-regulated the expression levels of Bax, Caspase-3, and PARP and up-regulated the expression level of Bcl2. Collectively, this study demonstrates that esculetin exerts potent antioxidant properties in ARPE-19 cells, inhibiting t -BHP-induced apoptosis under the regulation of apoptotic factors.
Keyphrases
- induced apoptosis
- oxidative stress
- endoplasmic reticulum stress
- cell cycle arrest
- signaling pathway
- diabetic rats
- cell death
- anti inflammatory
- dna damage
- poor prognosis
- endothelial cells
- ischemia reperfusion injury
- transcription factor
- high throughput
- cell proliferation
- long non coding rna
- smoking cessation
- drug induced
- combination therapy
- replacement therapy