Molecular Characterization and Phylogenetic Analysis of Fasciola gigantica Based on ITS-1 Genetic Marker and copro-epidemiological Survey from Ruminants of Pakistan.

The current study suggests that fascioliasis is a disease of primary concern indicating its status as one of the prevalent diseases affecting livestock in this region. It was also concluded that both Fasciola gigantica and Fasciola hepatica existed among the infected animals in addition to presence of hybrid/intermediate form in Pakistan with F. gigantica as dominant species in Punjab province.