A Chemically Inducible Muscle Ablation System for the Zebrafish.
Eric PaulissenBenjamin L MartinPublished in: Zebrafish (2024)
An effective method for tissue-specific ablation in zebrafish is the nitroreductase (NTR)/metronidazole (MTZ) system. Expressing bacterial NTR in the presence of nitroimidazole compounds causes apoptotic cell death, which can be useful for understanding many biological processes. However, this requires tissue-specific expression of the NTR enzyme, and many tissues have yet to be targeted with transgenic lines that express NTR. We generated a transgenic zebrafish line expressing NTR in differentiated skeletal muscle. Treatment of embryos with MTZ caused muscle specific cell ablation. We demonstrate this line can be used to monitor muscle regeneration in whole embryos and in transplanted transgenic cells.
Keyphrases
- skeletal muscle
- cell death
- cell cycle arrest
- insulin resistance
- induced apoptosis
- poor prognosis
- catheter ablation
- single cell
- gene expression
- type diabetes
- oxidative stress
- metabolic syndrome
- cell therapy
- signaling pathway
- adipose tissue
- drug delivery
- bone marrow
- cancer therapy
- endoplasmic reticulum stress
- cell proliferation
- atrial fibrillation
- combination therapy