Role of TRAF6 from obscure puffer (Takifugu obscurus) in immune response against Edwardsiella tarda infection.
Yan ShiXiaying LiangSufei HuMengMeng WangYifan WangZhe ZhaoPublished in: Journal of fish diseases (2023)
Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a vital molecule of inflammatory signaling pathways in innate immune response against pathogens. To elucidate its role in defense against Edwardsiella tarda infection in teleost fish, TRAF6 homologue was identified from obscure puffer (Takifugu obscurus) and functionally analyzed in this study. The obscure puffer TRAF6 (ToTRAF6) is a protein of 565 amino acids containing conserved RING domain, zinc finger-TRAF and MATH_TRAF6 domain. ToTRAF6 mRNA distributed in various healthy tissues of obscure puffer and was upregulated in the immune related tissues after E. tarda infection. ToTRAF6 protein was localized in the cytoplasm and aggregate as dots around the nuclei in FHM cells. The overexpression of ToTRAF6 in FHM cells decreased the quantity of E. tarda and induced the significant upregulation of downstream MAPK signaling pathway genes. These data suggest that ToTRAF6 is a key molecule of MAPK signaling pathway in defense against E. tarda infection.
Keyphrases
- signaling pathway
- induced apoptosis
- immune response
- pi k akt
- cell cycle arrest
- amino acid
- epithelial mesenchymal transition
- oxidative stress
- gene expression
- binding protein
- cell proliferation
- endoplasmic reticulum stress
- dendritic cells
- toll like receptor
- genome wide
- poor prognosis
- high glucose
- deep learning
- inflammatory response
- high resolution
- cell death
- endothelial cells
- molecularly imprinted