Role of IS Kpn23 in bla BKC-1 Expression and Mobilization.

This study aimed to verify the role of IS Kpn23 in the expression and mobilization of bla BKC-1 and aph(3')-VIi . Five constructs related to the natural bla BKC-1 genetic background in plasmid p60136 were made and submitted for antimicrobial susceptibility testing and quantitative reverse transcription-PCR. Transposition of IS Kpn23-bla BKC-1 was investigated using transposition assays involving a 9.7-kb nonconjugative plasmid carrying bla BKC-1 (p60136) and a transfer-proficient plasmid (pOX38-Gen). The presence of IS Kpn23 had a crucial role in bla BKC-1 expression, resulting in increased β-lactam MICs. While we detected mobilization of p60136 by the pOX38-Gen plasmid, transposition of IS Kpn23-bla BKC-1 was not observed.