New Insights into Bioactive Compounds from the Medicinal Plant Spathodea campanulata P. Beauv. and Their Activity against Helicobacter pylori.
Corinne Raïssa NgnamekoLucia MarchettiBarbara ZambelliAntonio QuotadamoDavide RoncaratiDavide BertelliFrederic Nico NjayouStella I SmithPaul F MoundipaMaria Paola CostiFederica PellatiPublished in: Antibiotics (Basel, Switzerland) (2020)
The medicinal plant Spathodea campanulata P. Beauv. (Bignoniaceae) has been traditionally applied for the prevention and treatment of diseases of the kidney and urinary system, the skin, the gastrointestinal tract, and inflammation in general. The present work shows for the first time how chemical components from this plant inhibit Helicobacter pylori growth by urease inhibition and modulation of virulence factors. The crude extract and the main fractions of S. campanulata bark were tested on H. pylori isolated strains and the active ones were further fractionated. Fractions and sub-fractions of the plant crude extract were characterized by ultra-high-performance liquid chromatographic tandem high resolution-mass spectrometry detection (UHPLC-HRMS). Several phenolics and triterpenoids were identified. Among the sub-fractions obtained, SB2 showed the capacity to inhibit H. pylori urease in a heterologous bacterial model. One additional sub-fraction (SE3) was able to simultaneously modulate the expression of two adhesins (HopZ and BabA) and one cytotoxin (CagA). The flavonol kaempferol was identified as the most interesting compound that deserves further investigation as a new hit for its capacity to modulate H. pylori virulence factors.
Keyphrases
- helicobacter pylori
- high resolution mass spectrometry
- helicobacter pylori infection
- escherichia coli
- liquid chromatography
- oxidative stress
- pseudomonas aeruginosa
- staphylococcus aureus
- ultra high performance liquid chromatography
- biofilm formation
- simultaneous determination
- poor prognosis
- antimicrobial resistance
- cell wall
- gas chromatography
- tandem mass spectrometry
- cystic fibrosis
- anti inflammatory
- high resolution
- small cell lung cancer
- candida albicans
- replacement therapy
- soft tissue