Gelatin-Polyaniline Composite Nanofibers Enhanced Excitation-Contraction Coupling System Maturation in Myotubes.
Serge OstrovidovMajid EbrahimiHojae BaeHung Kim NguyenSahar SalehiSang Bok KimAkichika KumataniTomokazu MatsueXuetao ShiKen NakajimaShizu HidemaMakoto OsanaiAli KhademhosseiniPublished in: ACS applied materials & interfaces (2017)
In this study, composite gelatin-polyaniline (PANI) nanofibers doped with camphorsulfonic acid (CSA) were fabricated by electrospinning and used as substrates to culture C2C12 myoblast cells. We observed enhanced myotube formation on composite gelatin-PANI nanofibers compared to gelatin nanofibers, concomitantly with enhanced myotube maturation. Thus, in myotubes, intracellular organization, colocalization of the dihydropyridine receptor (DHPR) and ryanodine receptor (RyR), expression of genes correlated to the excitation-contraction (E-C) coupling apparatus, calcium transients, and myotube contractibility were increased. Such composite material scaffolds combining topographical and electrically conductive cues may be useful to direct skeletal muscle cell organization and to improve cellular maturation, functionality, and tissue formation.
Keyphrases
- tissue engineering
- hyaluronic acid
- skeletal muscle
- bone regeneration
- reduced graphene oxide
- induced apoptosis
- poor prognosis
- smooth muscle
- insulin resistance
- single cell
- binding protein
- quantum dots
- cell therapy
- gene expression
- long non coding rna
- energy transfer
- oxidative stress
- mass spectrometry
- dna methylation
- cell death
- metabolic syndrome
- liquid chromatography
- tandem mass spectrometry