Identification of a Polymorphism in the N Gene of SARS-CoV-2 That Adversely Impacts Detection by Reverse Transcription-PCR.

Manu VanaerschotSabrina A MannJames T WebberJack KammSidney M BellJohn BellSi Noon HongMinh Phuong NguyenLienna Y ChanKaran D BhattMichelle TanAngela M DetweilerAlex EspinosaWesley WuJoshua BatsonDavid DynermanDebra A WadfordAndreas S PuschnikNorma NeffVida AhyongSteve MillerPatrick AyscueCristina M TatoSimon PaulAmy L KistlerJoseph L DeRisiEmily D Crawford

Published in: Journal of clinical microbiology (2020)

Since April 7, 2020, our COVID-19 diagnostic laboratory (CLIAHUB) has received samples from multiple counties in California - our RT-PCR protocol (1) employs N-gene (NIID_2019-nCov_N_F2/R2ver3/P2 (Japan) (2)) and E-gene (E_Sarbeco_F/R/P1 (Germany) (3)) simplex assays.….

Keyphrases

sars cov
copy number
genome wide
genome wide identification
real time pcr
coronavirus disease
randomized controlled trial
respiratory syndrome coronavirus
transcription factor
high throughput
genome wide analysis
quantum dots
sensitive detection