Biological activity of recombinant human PDX1 protein produced from Escherichia coli.
Gloria NarayanPlaboni SenShirisha NagotuRajkumar P ThummerPublished in: Journal of biochemical and molecular toxicology (2023)
Pancreatic and duodenum homeobox 1 (PDX1) is considered as a pivotal transcription factor that acts as a "master regulator" in pancreatogenesis and maintenance of β-cells. Earlier study has reported that PDX1 also functions as a tumor suppressor in human gastric cancer cells by inhibiting cell growth. Here, we report the bioactivity of the purified human PDX1 fusion protein using various assays like cell migration, proliferation, cell cycle analysis, and gene expression. In cancer cells, recombinant PDX1 protein reduced cell migration and proliferation, and arrested cell growth by inducing apoptosis in gastric cancer cells. In pancreatic ductal cancer cells, the application of the PDX1 protein resulted in the induction of insulin gene expression. The results of these experiments demonstrate the biological activity imparted by recombinant human PDX1 fusion protein on gastric and pancreatic cancer cells and its usefulness as a biological tool to elucidate its function in various cellular processes.
Keyphrases
- cell migration
- recombinant human
- gene expression
- cell cycle
- transcription factor
- escherichia coli
- endothelial cells
- signaling pathway
- cell cycle arrest
- cell proliferation
- induced apoptosis
- protein protein
- type diabetes
- oxidative stress
- amino acid
- induced pluripotent stem cells
- endoplasmic reticulum stress
- pluripotent stem cells
- high throughput
- small molecule
- pi k akt