Combining loss of function of FOLYLPOLYGLUTAMATE SYNTHETASE1 and CAFFEOYL-COA 3-O-METHYLTRANSFERASE1 for lignin reduction and improved saccharification efficiency in Arabidopsis thaliana.
Hongli XieNancy L EngleSivasankari VenketachalamChang Geun YooJaime BarrosMitch LecoultreNikki HowardGuifen LiLiang SunAvinash C SrivastavaSivakumar PattathilYunqiao PuMichael G HahnArthur J RagauskasRichard S NelsonRichard A DixonTimothy J TschaplinskiElison B BlancaflorYuhong TangPublished in: Biotechnology for biofuels (2019)
Our observations demonstrate that additional reduction in lignin content and improved sugar release can be achieved by simultaneous downregulation of a gene in the C1 (FPGS1) and lignin biosynthetic (CCOAOMT) pathways. These improvements in sugar accessibility were achieved without introducing unwanted long-term plant growth and developmental defects.