Dietary restriction promote sperm remodeling in aged roosters based on transcriptome analysis.
Wenjie LiangYuehua HeTingqi ZhuBinbin ZhangShuangxing LiuHaishan GuoPingquan LiuHuayuan LiuDonghua LiXiangtao KangWenting LiGui-Rong SunPublished in: BMC genomics (2024)
Compared with the 47DG group, sperm motility (P < 0.05), sperm density (P < 0.01), and testis weight (P < 0.05) were significantly increased in roosters in the D group. Further RNA-seq analysis of the testis between the D group and 47DG group identified 61 DEGs, with 21 up-regulated and 40 down-regulated. Functional enrichment analysis showed that the DEGs were primarily enriched in the cytokine-cytokine receptor interaction, Wnt signaling pathway, MAPK signaling pathway, TGF-β signaling pathway, and focal adhesion pathway. The qRT-PCR results showed that the expression trend of these genes was consistent with the sequencing results. WNT5A, FGFR3, AGTR2, TGFβ2, ROMO1, and SLC26A7 may play a role in testis development and spermatogenesis. This study provides fundamental data to enhance the reproductive value of aging roosters.
Keyphrases
- signaling pathway
- rna seq
- pi k akt
- single cell
- epithelial mesenchymal transition
- transforming growth factor
- cell proliferation
- induced apoptosis
- stem cells
- transcription factor
- poor prognosis
- physical activity
- biofilm formation
- weight loss
- electronic health record
- binding protein
- big data
- pseudomonas aeruginosa
- cystic fibrosis
- germ cell
- artificial intelligence
- weight gain
- deep learning