Promote Lipolysis in White Adipocytes by Magnetic Hyperthermia Therapy with Fe3O4 Microspheres Doped Hydrogel.

Obesity has become an ongoing global crisis, since it increases the risks of cardiovascular disease, type 2 diabetes, fatty liver, cognitive decline, and some cancer diseases. Adipose tissue is closely associated with the disorder of lipid metabolism. Several efforts had been made on modulation of lipid accumulation, but hindered by poor efficiency of cellular uptake, low safety, and uncertain effective dosage. Herein, we design a Fe3O4 microspheres doped composite hydrogel (Fe3O4 microspheres @chitosan/β-glycerophosphate/collagen), termed as Fe3O4@Gel, as the magnetocaloric agent for magnetic hyperthermia therapy (MHT), aiming to promote the lipolysis in white adipocytes. The experimental results show that the obtained Fe3O4@Gel displays a series of advantages, such as fast sol-gel transition, high biocompatibility, and excellent magneto-thermal performance. MHT, which is realized by Fe3O4@Gel subjected to alternating magnetic field (AMF), leads to reduced lipid accumulation, lower triglyceride content, and increased mitochondrial activity in white adipocytes. This work shows that Fe3O4@Gel-mediated MHT can effectively promote the lipolysis in white adipocytes in vitro, which provides a potential approach to treat obesity and associated metabolic disorders.