Sepia pharaonis Ink Mitigates Dehydroepiandrosterone-Induced Insulin Resistance in Mouse Model of Polycystic Ovarian Syndrome.
Prathyusha YamarthiRama Satyasri KotipalliSamatasai PatnaikKv VeenaMuralidharan KathirvelRajkumar VutukuriManjula BhanooriPublished in: Pathophysiology : the official journal of the International Society for Pathophysiology (2024)
The present study aims to evaluate the effect of Sepia pharaonis ink on insulin resistance in PCOS-induced mice. Treatment with sepia ink in dehydroepiandrosterone (DHEA)-induced PCOS mice at various doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg body weight mitigated the insulin resistance in the study groups with decreased concentration of testosterone and increased concentrations of estrogen and progesterone compared to the PCOS group tested by ELISA. The histopathological analysis and restoration of glucose analysis showed a significant reduction in treatment groups. Reduced expression of insulin resistance genes like androgen receptor (AR), insulin receptor substrate 1 (IRS-1), and insulin-like growth factor1 (IGF-1) by qRT-PCR indicate a positive impact of sepia ink in alleviating the symptoms associated with PCOS. Taken together, the results of this study indicate sepia ink as a promising therapeutic intervention and a possible drug target for insulin resistance in diabetes and gynecological disorders like PCOS.
Keyphrases
- polycystic ovary syndrome
- insulin resistance
- type diabetes
- body weight
- mouse model
- high glucose
- randomized controlled trial
- cardiovascular disease
- metabolic syndrome
- diabetic rats
- radiation therapy
- cell proliferation
- adipose tissue
- estrogen receptor
- skeletal muscle
- high fat diet
- blood pressure
- blood glucose
- transcription factor
- replacement therapy
- genome wide
- long non coding rna
- gene expression
- physical activity
- signaling pathway
- endothelial cells
- data analysis
- electronic health record
- adverse drug
- genome wide identification
- growth hormone
- genome wide analysis