Heat shock-induced enhanced susceptibility of barley to Bipolaris sorokiniana is associated with elevated ROS production and plant defense-related gene expression.

● Heat stress alters plant defense responses to pathogens. A short-term heat shock (HS) promotes infections by biotrophic pathogens. However, little is known about how HS affects infection by hemibiotrophic pathogens like Bipolaris sorokiniana (teleomorph: Cochliobolus sativus) (BS). We assessed the effect of HS in BS-susceptible barley (Hordeum vulgare cv. Ingrid) by monitoring leaf spot symptoms, BS biomass, reactive oxygen species (ROS) and plant defense-related gene expression following pre-exposure to HS. ● For HS, barley plants were kept at 49 °C for 20 seconds. BS biomass was assessed by qPCR, ROS levels determined by histochemical staining, while gene expression assayed by RT-qPCR. ● HS suppressed defense responses of barley to BS, resulting in more severe necrotic symptoms and increased fungal biomass, as compared to untreated plants. HS-induced increased susceptibility was accompanied by significant increases in ROS (superoxide, hydrogen peroxide). Transient expression of plant defense-related antioxidant genes and a barley programmed cell death inhibitor (HvBI-1) was induced in response to HS. However, HS followed by BS infection caused further transient increases in expression of HvSOD and HvBI-1 correlating with enhanced susceptibility. Expression of the HvPR-1b gene encoding pathogenesis-related (PR) protein-1b increased several fold at 24 hours after BS infection, however, HS further increased transcript levels along with enhanced susceptibility. ● HS induces enhanced susceptibility of barley to BS, associated with elevated ROS levels and expression of plant defense-related genes encoding antioxidants, a cell death inhibitor and PR-1b. Our results may contribute to elucidating the influence of HS on barley defense responses to hemibiotrophic pathogens.