Currently, there is no protocol for growing and culturing primary pulmonary arterial cells (PACs) available from the Pulmonary Hypertension Breakthrough Initiative (PHBI). Here, we present a protocol for cultivating and maintaining three major PACs collected from patients with pulmonary arterial hypertension (PAH): endothelial (PAH-ECs), smooth muscle (PAH-SMCs), and adventitial cells (PAH-ADCs). We describe steps for obtaining PACs from PHBI, evaluating the growth of cells labeled with quantum dots (QDs), and staining endothelial cell (EC) markers for immunofluorescence imaging. For complete details on the use and execution of this protocol, please refer to Al-Hilal et al. 1 .
Keyphrases
- pulmonary arterial hypertension
- pulmonary hypertension
- induced apoptosis
- pulmonary artery
- randomized controlled trial
- cell cycle arrest
- smooth muscle
- quantum dots
- endothelial cells
- polycyclic aromatic hydrocarbons
- endoplasmic reticulum stress
- high resolution
- signaling pathway
- cell death
- oxidative stress
- stem cells
- coronary artery
- quality improvement
- sensitive detection
- photodynamic therapy
- vascular endothelial growth factor
- pet imaging
- flow cytometry