Hibiscus Anthocyanins Extracts Induce Apoptosis by Activating AMP-Activated Protein Kinase in Human Colorectal Cancer Cells.
Ming-Chang TsaiChing-Chun ChenTsui-Hwa TsengYun-Ching ChangYi-Jie LinI-Ning TsaiChi-Chih WangChau-Jong WangPublished in: Nutrients (2023)
Apoptosis, a programmed cell death process preventing cancer development, can be evaded by cancer cells. AMP-activated protein kinase (AMPK) regulates energy levels and is a key research topic in cancer prevention and treatment. Some bioactive components of Hibiscus sabdariffa L. (HAs), including anthocyanins, have potential anticancer properties. Our study investigated the in vitro cytotoxic potential and mode of action of HAs extracts containing anthocyanins in colorectal cancer cells. The results showed that Hibiscus anthocyanin-rich extracts induced apoptosis in human colorectal cancer cells through the activation of multiple signaling pathways of AMPK. We observed the dose-response and time-dependent induction of apoptosis with HAs. Subsequently, the activation of Fas-mediated proteins triggered apoptotic pathways associated with Fas-mediated apoptosis-related proteins, including caspase-8/tBid. This caused the release of cytochrome C from the mitochondria, resulting in caspase-3 cleavage and apoptosis activation in intestinal cancer cells. These data elucidate the relationship between Has' regulation of apoptosis-related proteins in colorectal cancer cells and apoptotic pathways.
Keyphrases
- endoplasmic reticulum stress
- cell death
- induced apoptosis
- protein kinase
- cell cycle arrest
- oxidative stress
- signaling pathway
- endothelial cells
- pi k akt
- squamous cell
- young adults
- risk assessment
- human health
- climate change
- anti inflammatory
- epithelial mesenchymal transition
- mass spectrometry
- pluripotent stem cells
- data analysis