Selenium Nanoparticles Attenuate Cobalt Nanoparticle-Induced Skeletal Muscle Injury: A Study Based on Myoblasts and Zebrafish.
Zejiu TanLinhua DengZhongjing JiangGang XiangGengming ZhangSihan HeHong-Qi ZhangYunjia WangPublished in: Toxics (2024)
Cobalt alloys have numerous applications, especially as critical components in orthopedic biomedical implants. However, recent investigations have revealed potential hazards associated with the release of nanoparticles from cobalt-based implants during implantation. This can lead to their accumulation and migration within the body, resulting in adverse reactions such as organ toxicity. Despite being a primary interface for cobalt nanoparticle (CoNP) exposure, skeletal muscle lacks comprehensive long-term impact studies. This study evaluated whether selenium nanoparticles (SeNPs) could mitigate CoNP toxicity in muscle cells and zebrafish models. CoNPs dose-dependently reduced C2C12 viability while elevating reactive oxygen species (ROS) and apoptosis. However, low-dose SeNPs attenuated these adverse effects. CoNPs downregulated myogenic genes and α-smooth muscle actin (α-SMA) expression in C2C12 cells; this effect was attenuated by SeNP cotreatment. Zebrafish studies confirmed CoNP toxicity, as it decreased locomotor performance while inducing muscle injury, ROS generation, malformations, and mortality. However, SeNPs alleviated these detrimental effects. Overall, SeNPs mitigated CoNP-mediated cytotoxicity in muscle cells and tissue through antioxidative and antiapoptotic mechanisms. This suggests that SeNP-coated implants could be developed to eliminate cobalt nanoparticle toxicity and enhance the safety of metallic implants.
Keyphrases
- skeletal muscle
- cell cycle arrest
- induced apoptosis
- oxidative stress
- cell death
- reactive oxygen species
- low dose
- smooth muscle
- endoplasmic reticulum stress
- insulin resistance
- reduced graphene oxide
- dna damage
- carbon nanotubes
- metal organic framework
- soft tissue
- type diabetes
- poor prognosis
- metabolic syndrome
- endothelial cells
- gold nanoparticles
- spinal cord injury
- case control
- iron oxide
- high glucose
- anti inflammatory
- electronic health record