Hypsizygus marmoreus extract exhibited antioxidant effects to promote longevity and stress resistance in Caenorhabditis elegans .

In this study, we explored the lifespan extension effect of a popular edible mushroom, Hypsizygus marmoreus , using the model organism Caenorhabditis elegans ( C. elegans ). The results showed that Hypsizygus marmoreus extract (HME) could increase the lifespan of C. elegans and ameliorate the healthspan by improving motility, attenuating lipofuscin accumulation, and enhancing the ability to withstand oxidative and heat stress. Then, we found noteworthy enhancements in SOD and CAT activities and reactive oxygen species (ROS) scavenging activity in vivo . Combined with the up-regulation of the expression of antioxidant genes ( skn-1, sod-1 , sod-3 , mev-1 , and gst-4 ), HME may function as an antioxidant in nematodes, which may be closely related to its phenolic compounds. Furthermore, we found that HME promoted the transfer of the transcription factor SKN-1 to the nucleus but had no impact on the lifespan of skn-1 mutants, indicating that SKN-1 was essential for Hypsizygus marmoreus to exert beneficial biological effects in C. elegans . Our findings elucidated that dietary supplementation with Hypsizygus marmoreus might have beneficial anti-aging effects and contribute to exploring the lifespan extension and underlying mechanisms of Hypsizygus marmoreus in C. elegans .