Simple and low-cost antibiotic susceptibility testing for Mycobacterium tuberculosis using screen-printed electrodes.
Hamed GhorbanpoorIremnur AkcakocaAraz Norouz DizajiAdrian ButterworthDamion CorriganTanil KocagozAliakbar EbrahimiHuseyin AvciFatma Dogan GuzelPublished in: Biotechnology and applied biochemistry (2023)
One quarter of the global population is thought to be latently infected by Mycobacterium tuberculosis (TB) with it estimated that 1 in 10 of those people will go on to develop active disease. Due to the fact that M. tuberculosis (TB) is a disease most often associated with low- and middle-income countries, it is critical that low-cost and easy-to-use technological solutions are developed, which can have a direct impact on diagnosis and prescribing practice for TB. One area where intervention could be particularly useful is antibiotic susceptibility testing (AST). This work presents a low-cost, simple-to-use AST sensor that can detect drug susceptibility on the basis of changing RNA abundance for the typically slow-growing M. tuberculosis (TB) pathogen in 96 h using screen-printed electrodes and standard molecular biology laboratory reactionware. In order to find out the sensitivity of applied sensor platform, a different concentration (10 8 -10 3 CFU/mL) of M. tuberculosis was performed, and limit of detection and limit of quantitation were calculated as 10 3.82 and 10 11.59 CFU/mL, respectively. The results display that it was possible to detect TB sequences and distinguish antibiotic-treated cells from untreated cells with a label-free molecular detection. These findings pave the way for the development of a comprehensive, low-cost, and simple-to-use AST system for prescribing in TB and multidrug-resistant tuberculosis.
Keyphrases
- low cost
- mycobacterium tuberculosis
- pulmonary tuberculosis
- label free
- primary care
- multidrug resistant
- high throughput
- randomized controlled trial
- induced apoptosis
- ms ms
- reduced graphene oxide
- cell proliferation
- cell cycle arrest
- hepatitis c virus
- gold nanoparticles
- wastewater treatment
- human immunodeficiency virus
- microbial community
- tandem mass spectrometry
- acinetobacter baumannii
- sensitive detection
- high resolution
- hiv infected
- newly diagnosed
- antiretroviral therapy