Fibronectin Type III Domain Containing 3B as a Potential Prognostic and Therapeutic Biomarker for Glioblastoma.
Hyukjun KwonMinji YunTaek-Hyun KwonMinji BangJungsul LeeYeo Song LeeHae Young KoKyuha ChongPublished in: Biomedicines (2023)
Glioblastoma (GBM) is a representative malignant brain tumor characterized by a dismal prognosis, with survival rates of less than 2 years and high recurrence rates. Despite surgical resection and several alternative treatments, GBM remains a refractory disease due to its aggressive invasiveness and resistance to anticancer therapy. In this report, we explore the role of fibronectin type III domain containing 3B (FNDC3B) and its potential as a prognostic and therapeutic biomarker in GBM. GBM exhibited a significantly higher cancer-to-normal ratio compared to other organs, and patients with high FNDC3B expression had a poor prognosis ( p < 0.01). In vitro studies revealed that silencing FNDC3B significantly reduced the expression of Survivin, an apoptosis inhibitor, and also reduced cell migration, invasion, extracellular matrix adhesion ability, and stem cell properties in GBM cells. Furthermore, we identified that FNDC3B regulates PTEN/PI3K/Akt signaling in GBM cells using MetaCore integrated pathway bioinformatics analysis and a proteome profiler phospho-kinase array with sequential western blot analysis. Collectively, our findings suggest FNDC3B as a potential biomarker for predicting GBM patient survival and for the development of treatment strategies for GBM.
Keyphrases
- poor prognosis
- type iii
- cell cycle arrest
- pi k akt
- cell migration
- long non coding rna
- induced apoptosis
- cell death
- stem cells
- signaling pathway
- extracellular matrix
- cell proliferation
- endoplasmic reticulum stress
- oxidative stress
- south africa
- bioinformatics analysis
- squamous cell carcinoma
- binding protein
- tyrosine kinase
- cystic fibrosis
- bone marrow
- papillary thyroid