The draft genome sequence of Scheffersomyces segobiensis strain DSM 27193, a yeast capable of producing palmitoleic acid-rich lipids.
Xinhai ZhouLijie XuDawei ZhouJie ZhouFengxue XinWenming ZhangXiujuan QianWeiliang DongMin JiangKatrin OchsenreitherPublished in: 3 Biotech (2021)
In this study, the draft genome of the recently isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193, which can accumulate high content of palmitoleic acid (POA), was sequenced and analyzed. Only few studies have reported about POA-rich lipid production by Scheffersomyces segobiensis so far. The ITS region analysis indicated that strain DSM 27193 is closely related to Pichia segobiensis and Scheffersomyces stipitis. The size of the assembled draft genome of strain DSM 27193 is 14.8 Mb, containing 5477 encoded protein sequences with a G + C content of 40.83%. Among the annotated genes, two stearoyl-CoA desaturases encoded by ole1 and ole2 were identified which are potentially involved in POA accumulation. Further analysis of POA-rich lipid synthesis pathway genes in S. segobiensis DSM 27193 will provide additional insights for POA artificial synthesis through metabolic engineering.