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More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display.

Aleksandra ŠakanovićNace KranjcNeža OmersaMarjetka PodobnikGregor Anderluh
Published in: RSC advances (2020)
Herein, we report a high-throughput approach for the selection of peripheral protein domains that bind specifically to cholesterol in lipid membranes. We discovered variants of perfringolysin O, with non-conserved amino acid substitutions at regions crucial for cholesterol recognition, demonstrating an unprecedented amino acid sequence variability with binding ability for cholesterol. The developed approach provides an effective platform for a comprehensive study of protein lipid interactions.
Keyphrases
  • amino acid
  • low density lipoprotein
  • high throughput
  • binding protein
  • fatty acid
  • transcription factor
  • protein protein
  • gene expression
  • single cell
  • dna binding
  • small molecule