Improving negative liquid chromatography/electrospray ionization mass spectrometry lipidomic analysis of human plasma using acetic acid as a mobile-phase additive.

Acetic acid at a concentration of 0.02% (v/v) is the suggested choice as a mobile-phase additive for lipidomics and targeted lipid profiling with negative LC/ESI-MS based on signal enhancement and improved lipid coverage compared to ammonium acetate, ammonium acetate with acetic acid and ammonium hydroxide mobile phases.