Anti-inflammatory Role of Carotenoids in Endothelial Cells Derived from Umbilical Cord of Women Affected by Gestational Diabetes Mellitus.
Mariangela UcciPamela Di TomoFederica TritschlerVincenzo G P CordonePaola LanutiGiuseppina BolognaSara Di SilvestreNatalia Di PietroCaterina PipinoDomitilla MandatoriGloria FormosoAssunta PandolfiPublished in: Oxidative medicine and cellular longevity (2019)
Diabetes is associated with vascular inflammation, endothelial dysfunction, and oxidative stress, promoting the development of cardiovascular diseases (CVD). Several studies showed that a carotenoid-rich diet is associated to a reduced cardiovascular risk in healthy and diabetic subjects, although the mechanisms of action are still unknown. Here, the potential role of β-carotene (BC) and lycopene (Lyc) in human endothelial cells isolated from human umbilical cord vein (HUVECs) of women with gestational diabetes (GD) and respective controls (C) has been investigated. Results showed that BC and Lyc reduced the tumor necrosis factor alpha- (TNF-α-) stimulated monocyte-endothelium interaction (adhesion assay), membrane exposure (flow cytometry), and total expression levels (Western blot) of VCAM-1 and ICAM-1 in both cell types. Moreover, the treatment with BC and Lyc reduced the TNF-α-induced nuclear translocation of NF-κB (image flow cytometry) by preserving bioavailability of nitric oxide (NO, flow cytometry, and cGMP EIA kit assay), a key vasoactive molecule. Notably, BC and Lyc pretreatment significantly reduced peroxynitrite levels (flow cytometry), contributing to the redox balance protection. These results suggest a new mechanism of action of carotenoids which exert vascular protective action in diabetic condition, thus reinforcing the importance of a carotenoid-rich diet in the prevention of diabetes cardiovascular complications.
Keyphrases
- flow cytometry
- endothelial cells
- umbilical cord
- high glucose
- mesenchymal stem cells
- nitric oxide
- oxidative stress
- type diabetes
- cardiovascular disease
- rheumatoid arthritis
- diabetic rats
- vascular endothelial growth factor
- pregnancy outcomes
- anti inflammatory
- weight loss
- high throughput
- glycemic control
- nitric oxide synthase
- stem cells
- physical activity
- dna damage
- poor prognosis
- deep learning
- lps induced
- hydrogen peroxide
- signaling pathway
- bone marrow
- cell therapy
- polycystic ovary syndrome
- binding protein
- induced pluripotent stem cells
- risk factors
- wound healing
- staphylococcus aureus
- mass spectrometry
- pseudomonas aeruginosa
- ischemia reperfusion injury
- induced apoptosis
- adipose tissue
- long non coding rna
- replacement therapy
- cardiovascular risk factors
- skeletal muscle
- escherichia coli
- metabolic syndrome
- toll like receptor
- insulin resistance
- drug induced
- pi k akt