Immunomagnetic Selection of Plasmodium falciparum-Infected Erythrocytes Expressing Particular PfEMP1 Variants.

Mary Lopez-Perez Rebecca Wendelboe Olsen

Published in: Methods in molecular biology (Clifton, N.J.) (2022)

Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.

Keyphrases

plasmodium falciparum
flow cytometry
randomized controlled trial
binding protein
gene expression
amino acid
wild type
data analysis