Ion mobility-resolved phosphoproteomics with dia-PASEF and short gradients.
Denys OliinykFlorian MeierPublished in: Proteomics (2022)
Mass spectrometry-based phosphoproteomics has identified >150,000 post-translational phosphorylation sites in the human proteome. To disentangle their functional relevance, complex experimental designs that require increased throughput are now coming into focus. Here, we apply dia-PASEF on a trapped ion mobility (TIMS) mass spectrometer to analyze the phosphoproteome of a human cancer cell line in short liquid chromatography gradients. At low sample amounts equivalent to ∼20 ug protein digest per analysis, we quantified over 13,000 phosphopeptides including ∼8700 class I phosphosites in 1 h without a spectral library. Decreasing the gradient time to 15 min yielded virtually identical coverage of the phosphoproteome, and with 7 min gradients we still quantified about 80% of the class I sites with a median coefficient of variation <10% in quadruplicates. We attribute this in part to the increased peak capacity, which effectively compensates for the higher peptide density per time unit in shorter gradients. Our data show a five-fold reduction in the number of co-isolated peptides with TIMS. In the most extreme case, these were positional isomers of nearby phosphosites that remained unresolved with fast liquid chromatography. In summary, our study demonstrates how key features of dia-PASEF translate to phosphoproteomics.
Keyphrases
- liquid chromatography
- mass spectrometry
- endothelial cells
- high resolution mass spectrometry
- tandem mass spectrometry
- high resolution
- induced pluripotent stem cells
- pluripotent stem cells
- high performance liquid chromatography
- gas chromatography
- capillary electrophoresis
- magnetic resonance
- solid phase extraction
- electronic health record
- climate change
- machine learning
- magnetic resonance imaging
- young adults
- computed tomography
- small molecule
- squamous cell
- healthcare
- big data
- squamous cell carcinoma
- protein kinase