Gene Expression Profiling of Apoptotic Proteins in Circulating Peripheral Blood Mononuclear Cells in Type II Diabetes Mellitus and Modulation by Metformin.

Mohammed Al DubayeeAwad AlshahraniDana AljadaMahmoud ZahraAhmed Alotaibi Ibrahim AbabtainMalik AlnaimAli Alahmari Abdullah AljarallahMuhammad Affan ElahiHana M A Fakhoury

Published in: Diabetes, metabolic syndrome and obesity : targets and therapy (2021)

The moderate up-regulation of pro-apoptotic Bcl-10, Bax, Bad, Bid, and the effector caspase-3 coupled with inhibition of caspase-7 in circulating PBMCs of T2DM could be the result of increased inflammation in T2DM. Metformin treatment significantly inhibited the expression of Bcl-10, Bid, and caspase-3 and upregulated Bad/Bax/caspase-7 pathway suggesting the activation of Bad/Bax/caspase-7 apoptotic pathway. Further studies are warranted to elicit the underlying apoptotic pathways of PBMCs in T2DM and following metformin treatment.

Keyphrases

cell death
induced apoptosis
gene expression
oxidative stress
anti inflammatory
signaling pathway
glycemic control
poor prognosis
type diabetes
metabolic syndrome
regulatory t cells
long non coding rna
insulin resistance
replacement therapy
weight loss