Ethanolic Extract of Artemisia vulgaris Leaf Promotes Apoptotic Cell Death in Non-Small-Cell Lung Carcinoma A549 Cells through Inhibition of the Wnt Signaling Pathway.
Rohit Kumar TiwariAfza AhmadAhamad Faiz KhanLamya Ahmed Al-KeridisMohd SaeedNawaf AlshammariNadiyah M AlabdallahIrfan Ahmad AnsariFarina MujeebPublished in: Metabolites (2023)
The Wnt signaling pathway is reported to be associated with lung cancer progression, metastasis and drug resistance, and thus it is an important therapeutic target for lung cancer. Plants have been shown as reservoirs of multiple potential anticancer agents. In the present investigation, the ethanolic leaf extract of Artemisia vulgaris ( AvL -EtOH) was initially analyzed by means of gas chromatography-mass spectrometry (GC-MS) to identify the important phytochemical constituents. The GC-MS analysis of AvL -EtOH exhibited 48 peaks of various secondary metabolites such as terpenoids, flavonoids, carbohydrates, coumarins, amino acids, steroids, proteins, phytosterols, and diterpenes. It was found that the treatment with increasing doses of AvL -EtOH suppressed the proliferation and migration of lung cancer cells. Furthermore, AvL -EtOH induced prominent nuclear alteration along with a reduction in mitochondrial membrane potential and increased ROS (reactive oxygen species) generation in lung cancer cells. Moreover, AvL -EtOH-treated cells exhibited increased apoptosis, demonstrated by the activation of caspase cascade. AvL -EtOH also induced downregulation of Wnt3 and β-catenin expression along with cell cycle protein cyclin D1. Thus, the results of our study elucidated the potential of bioactive components of Artemisia vulgaris in the therapeutic management of lung cancer cells.
Keyphrases
- cell cycle arrest
- cell death
- cell proliferation
- pi k akt
- cell cycle
- induced apoptosis
- signaling pathway
- oxidative stress
- endoplasmic reticulum stress
- reactive oxygen species
- diabetic rats
- gas chromatography mass spectrometry
- stem cells
- amino acid
- epithelial mesenchymal transition
- poor prognosis
- human health
- binding protein
- drug induced
- combination therapy
- cell therapy
- anti inflammatory
- mesenchymal stem cells
- dna damage
- single cell
- ms ms
- newly diagnosed
- mass spectrometry
- smoking cessation
- stress induced
- climate change
- replacement therapy