Network Analyses of Differentially Expressed Genes in Osteoarthritis to Identify Hub Genes.
Zhaoyan LiLei ZhongZhenwu DuGaoyang ChenJing ShangQiwei YangQingyu WangYang SongGuizhen ZhangPublished in: BioMed research international (2019)
A total of 229 DEGs were identified in osteoarthritis synovial membranes compared with normal synovial membranes, including 145 upregulated and 84 downregulated differentially expressed genes. The KEGG pathway analysis results showed that up-DEGs were enriched in proteoglycans in cytokine-cytokine receptor interaction, chemokine signaling pathway, rheumatoid arthritis, and TNF signaling pathway, whereas down-DEGs were enriched in the PPAR signaling pathway and AMPK signaling pathway. The qRT-PCR results showed that the expression levels of ADIPOQ, IL6, and CXCR1 in the synovium of osteoarthritis were significantly increased (p <0.05).
Keyphrases
- signaling pathway
- rheumatoid arthritis
- pi k akt
- epithelial mesenchymal transition
- bioinformatics analysis
- genome wide
- induced apoptosis
- disease activity
- knee osteoarthritis
- genome wide identification
- poor prognosis
- interstitial lung disease
- insulin resistance
- skeletal muscle
- network analysis
- dna methylation
- gene expression
- type diabetes
- oxidative stress
- transcription factor
- cell migration