Acute and short-term administrations of delta-9-tetrahydrocannabinol modulate major gut metabolomic regulatory pathways in C57BL/6 mice.
Megha OzaWilliam BeckerPhani M GummadidalaTravis DiasMayomi H OmebeyinjeLi ChenChandrani MitraRubaiya JesminParamita ChakrabortyMathew SajishLorne J HofsethKoyeli BanerjeeQian WangPeter D R MoellerMitzi NagarkattiPrakash NagarkattiAnindya ChandaPublished in: Scientific reports (2019)
Delta-9-tetrahydrocannabinol (THC) is the primary psychoactive compound in Cannabis, which is studied extensively for its medicinal value. A central gap in the science is the underlying mechanisms surrounding THC's therapeutic effects and the role of gut metabolite profiles. Using a mass-spectrometry based metabolomics, we show here that intraperitoneal injection of THC in C57BL/6 mice modulates metabolic profiles that have previously been identified as integral to health. Specifically, we investigated the effects of acute (single THC injection denoted here as '1X') and short -term (five THC injections on alternate days denoted as '5X') THC administration on fecal and intestinal tissue metabolite profiles. Results are consistent with the hypothesis that THC administration alters host metabolism by targeting two prominent lipid metabolism pathways: glycerophospholipid metabolism and fatty acid biosynthesis.
Keyphrases
- mass spectrometry
- fatty acid
- liver failure
- public health
- healthcare
- respiratory failure
- mental health
- drug induced
- high fat diet induced
- high resolution
- multidrug resistant
- transcription factor
- metabolic syndrome
- type diabetes
- liquid chromatography
- skeletal muscle
- intensive care unit
- atomic force microscopy
- capillary electrophoresis
- high speed