Improved and expanded Q-system reagents for genetic manipulations.

Olena RiabininaDavid LuginbuhlElizabeth MarrSha LiuMark N WuLiqun LuoChristopher J Potter

Published in: Nature methods (2015)

The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.

Keyphrases

drosophila melanogaster
poor prognosis
gene expression
binding protein
transcription factor
copy number
cell therapy
genome wide
stem cells
ionic liquid
oxidative stress
mesenchymal stem cells
immune response