Plasma membrane Ca2+ -ATPase 1 is required for maintaining atrial Ca2+ homeostasis and electrophysiological stability in the mouse.
Yanwen WangClaire WilsonElizabeth J CartwrightMing LeiPublished in: The Journal of physiology (2017)
To determine the role of plasma membrane Ca2+ -ATPase 1 (PMCA1) in maintaining Ca2+ homeostasis and electrical stability in the atrium under physiological and stress conditions, mice with a cardiomyocyte-specific deletion of PMCA1 (PMCA1cko ) and their control littermates (PMCA1loxP/loxP ) were studied at the organ and cellular levels. At the organ level, the PMCA1cko hearts became more susceptible to atrial arrhythmias under rapid programmed electrical stimulation compared with the PMCA1loxP/loxP hearts, and such arrhythmic events became more severe under Ca2+ overload conditions. At the cellular level, the occurrence of irregular-type action potentials of PMCA1cko atrial myocytes increased significantly under Ca2+ overload conditions and/or at higher frequency of stimulation. The decay of Na+ /Ca2+ exchanger current that followed a stimulation protocol was significantly prolonged in PMCA1cko atrial myocytes under basal conditions, with Ca2+ overload leading to even greater prolongation. In conclusion, PMCA1 is required for maintaining Ca2+ homeostasis and electrical stability in the atrium. This is particularly critical during fast removal of Ca2+ from the cytosol, which is required under stress conditions.
Keyphrases
- protein kinase
- atrial fibrillation
- left atrial
- heart failure
- spinal cord injury
- randomized controlled trial
- metabolic syndrome
- early onset
- risk assessment
- left ventricular
- angiotensin ii
- catheter ablation
- insulin resistance
- coronary artery
- inferior vena cava
- pulmonary arterial hypertension
- pulmonary artery
- left atrial appendage
- endothelial cells
- vena cava