Effects of isoflurane anaesthesia depth and duration on renal function measured with [ 99m Tc]Tc-mercaptoacetyltriglycine SPECT in mice.

Deep anaesthesia and prolonged low anaesthesia should be avoided during renal scintigraphy because they result in prolonged blood clearance half-life, delayed renal influx and/or later Tmax. Vice versa, low anaesthesia with high respiratory rates of 80-90 rpm and short duration (≤ 20 min) should be preferred to obtain representative data with low variance.