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Genomics-Driven Discovery of Benzoxazole Alkaloids from the Marine-Derived Micromonospora sp. SCSIO 07395.

Ziqian ChengQingbo ZhangJing PengXiaoyang ZhaoLiang MaChangsheng ZhangYiguang Zhu
Published in: Molecules (Basel, Switzerland) (2023)
Benzoxazole alkaloids exhibit a diverse array of structures and interesting biological activities. Herein we report the identification of a benzoxazole alkaloid-encoding biosynthetic gene cluster ( mich BGC) in the marine-derived actinomycete Micromonospora sp. SCSIO 07395 and the heterologous expression of this BGC in Streptomyces albus . This approach led to the discovery of five new benzoxazole alkaloids microechmycin A-E ( 1 - 5 ), and a previously synthesized compound 6 . Their structures were elucidated by HRESIMS and 1D and 2D NMR data. Microechmycin A (1) showed moderate antibacterial activity against Micrococcus luteus SCSIO ML01 with the minimal inhibitory concentration (MIC) value of 8 μg mL -1 .
Keyphrases
  • high resolution
  • high throughput
  • small molecule
  • poor prognosis
  • magnetic resonance
  • genome wide
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  • copy number
  • gene expression
  • solid state
  • genome wide identification
  • bioinformatics analysis