Engineering the substrate binding site of the hyperthermostable archaeal endo-β-1,4-galactanase from Ignisphaera aggregans.
Sebastian J MuderspachFolmer FredslundVerena VolfJens-Christian Navarro PoulsenThomas H BlicherMads Hartvig ClausenKim Krighaar RasmussenKristian B R M KroghKenneth JensenLeila Lo LeggioPublished in: Biotechnology for biofuels (2021)
We have characterized in detail the most thermophilic endo-β-1,4-galactanase known to date and successfully engineered it to modify the degradation profile, while maintaining much of its desirable thermostability. This is an important achievement as oligosaccharide products length is an important property for industrial and natural GHs alike.