Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues.
Adam K GlaserNicholas P RederYe ChenChengbo YinLinpeng WeiSoyoung KangLindsey A BarnerWeisi XieErin F McCartyChenyi MaoAaron R HalpernCaleb R StoltzfusJonathan S DanielsMichael Y GernerPhilip R NicovichJoshua C VaughanLawrence D TrueJonathan T C LiuPublished in: Nature communications (2019)
Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilitate wide adoption by preclinical researchers and clinical laboratories. In particular, the open-top geometry provides unsurpassed versatility to interface with a wide range of accessory technologies in the future.