Mitochondrial Dysfunction in Lung Resident Mesenchymal Stem Cells from Idiopathic Pulmonary Fibrosis Patients.
Josep Mercader BarcelóAina Martín-MedinaJoan Truyols-VivesGabriel Escarrer-GarauLinda Elowsson RendinAna Montes-WorboysCarlos Río-BocosJosep Muncunill-FarrenyJulio Velasco-RocaAnna CederbergMåns KadeforsMaria Molina-MolinaGunilla Westergren-ThorssonErnest Sala-LlinàsPublished in: Cells (2023)
Idiopathic pulmonary fibrosis (IPF) is characterized by an aberrant repair response with uncontrolled turnover of extracellular matrix involving mesenchymal cell phenotypes, where lung resident mesenchymal stem cells (LRMSC) have been supposed to have an important role. However, the contribution of LRMSC in lung fibrosis is not fully understood, and the role of LRMSC in IPF remains to be elucidated. Here, we performed transcriptomic and functional analyses on LRMSC isolated from IPF and control patients (CON). Both over-representation and gene set enrichment analyses indicated that oxidative phosphorylation is the major dysregulated pathway in IPF LRMSC. The most relevant differences in biological processes included complement activation, mesenchyme development, and aerobic electron transport chain. Compared to CON LRMSC, IPF cells displayed impaired mitochondrial respiration, lower expression of genes involved in mitochondrial dynamics, and dysmorphic mitochondria. These changes were linked to an impaired autophagic response and a lower mRNA expression of pro-apoptotic genes. In addition, IPF TGFβ-exposed LRMSC presented different expression profiles of mitochondrial-related genes compared to CON TGFβ-treated cells, suggesting that TGFβ reinforces mitochondrial dysfunction. In conclusion, these results suggest that mitochondrial dysfunction is a major event in LRMSC and that their occurrence might limit LRMSC function, thereby contributing to IPF development.
Keyphrases
- idiopathic pulmonary fibrosis
- mesenchymal stem cells
- interstitial lung disease
- end stage renal disease
- induced apoptosis
- newly diagnosed
- extracellular matrix
- cell death
- oxidative stress
- ejection fraction
- chronic kidney disease
- cell cycle arrest
- peritoneal dialysis
- bone marrow
- transforming growth factor
- stem cells
- genome wide
- poor prognosis
- patient reported outcomes
- risk assessment
- patient safety
- umbilical cord
- quality improvement
- signaling pathway
- endoplasmic reticulum stress
- epithelial mesenchymal transition
- copy number
- cell proliferation
- patient reported
- liver fibrosis